Safe Harbor Locus Master iPSC Genome Editing Service
Custom “Master” iPSC Cell Line Generation using TARGATT™ technology! The TARGATT™ Master Human iPSC Line contains an attP “docking site” at the hROSA26 safe harbor genomic locus. Any gene of interest can be stably inserted at the docking site using a corresponding “attB” containing TARGATT™ donor plasmid, with guaranteed gene expression. The efficiency of this phiC31 integrase-mediated recombination is up to 100% efficiency with drug selection and up to 30% without drug selection.
Our cell line model generation experts can also engineer your safe harbor locus knock-in iPSC lines using CRISPR/Cas9 for a multi-technology approach to generate a cell line model for your specific research needs.
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ASC engineered Site-Specific Knock-in of TARGATT™ sites, with mCherry reporter (left). Original Cell Line: hiPSC (right).
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Safe harbor locus master cell lines are ideal for applications using reporter lines for cell tracking, for cell purification using surface markers, and for directed differentiation of iPSCs using cell-specific promoters.
iPSC lines with cell-specific promoters:
Express cell-type specific transcription factors to induce differentiation of specific cell lineages
Reporters to track cell differentiation process
iPSC lines with excisable Cas9 or nickase for efficient CRISPR gene modification
High-efficiency, high purity cells differentiated from iPSC lines
Applied StemCell can also create Site-Specific Knock-in cell lines for primary cells or other cell lines.