CRISPR Cre-Rat With Gad67-CreERT2 Promoter (Rat)

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MTA required. The Gad67-CreERT2 rat model was generated using CRISPR/Cas9 for use as a breeding mate for conditional knockout/ knock-in rat models. To achieve this, a pair of in vivo validated gRNAs, a single-stranded deoxyribonucleic acid (ssDNA) donor, and the Cas9 protein were injected into the mouse embryos. Mice born from the injection were screened by genotyping PCR and NGS/ Sanger sequencing to confirm founders (F0). The Cre rat line generated expresses Cre recombinase under the control of tissue-specific gene promoter Gad67. Furthermore, DNA recombinase activity is induced by Tamoxifen, so that the Cre-Lox model can be both conditional and inducible.


Rat line



GABAergic neurons, islet cells and spermatocytes



10.2-kb containing 8.4-kb promoter and noncoding exon 0A and 0B of mouse glutamate decarboxylase 67

Kabayashi et al 2003

Rasmussen et al 2007