Human Fibroblast Immortalization Kit
Primary cells undergo a finite number of cell divisions in culture, after which they enter a state of senescence and no longer divide. To circumvent replicative senescence, the Simian virus 40 (SV40) T antigen is introduced to primary cells. The Simian virus 40 (SV40) is used as a reliable agent for the immortalization of different cell types and the mechanism of the SV40 T antigen in cell immortalization is well understood. Recent studies have also shown that SV40 T antigen can induce Telomerase activity in the infected cells.
Applied StemCell’s Human Fibroblast Immortalization Kits include an Immortalization Reagent (packaged lentivector constructs provided as frozen VSV-G pseudotyped viral particles) and a Virus Transduction Enhancer which can increase transduction rate up to 10-fold.