PD-1 & CTLA4

Reference the resources below for more information about the product/service or contact us today to learn more.

Strain Information

  • Catalog Number ASHU-00079
  • Model Name PD-1 & CTLA4
  • Strain Name C57BL/6 - Pdcd1em1(hPDCD1)Ctla4em1(hCTLA4)/Asc
  • Mutation Type Humanized
  • GENE Synonyms PD-1, Pdc1, Ly101, Cd152, Ly-56, Ctla-4
  • Human Orthologs PDCD1, CTLA4
  • Emsamble ID ENSMUSG00000026285, ENSMUSG00000026011
  • MGI ID 104879, 88556
  • NCBI ID 18566,12477
  • Background C57BL/6
  • Coat Color Black
  • Mutation Type Humanized
Animal Request Form Inquire
Catalog # :
Availability :
  • Description
  • Research Application
  • Related Products

Generation Strategy

Strategy for Generating Humanized PD-1 (ASHU-00015) Mouse Model


Strategy for Generating Humanized CTLA4 (ASHU-00014) Mouse Model


The Expression of hPD-1 in Stimulated T Cells from Double Humanized PD-1 & CTLA4 Mice 



Figure 1. The expression of human CTLA-4 in double humanized PD-1 & CTLA-4 mice was confirmed by FACS.


Figure 2. In vivo validation of double humanized PD-1 & CTLA-4 mice. The Double humanized mice were inoculated with MC38 cells, and randomly assigned to different groups (n=8) when the tumor grew to a volume of 100 mm3. A combinatorial treatment of anti-CTLA-4 and anti-PD-1 demonstrated a noticeable improvement in efficacy as compared to the same dose of single agent (top figure) without affecting the animal body weight (bottom).


Figure 3. As shown in the previous figure, animals of group 1-4 received different doses of either single agent or combinatorial treatment. This graph showed the progression of the tumor implanted in the cured, G4 mice, implicating a systematic anti-tumor effect induced by the combinatorial antibody treatment. 

Immunotherapy, cancer research, drug screening