• Rat Models

Rat Models

With the need to physiologically relevant animals models on the rise for more predictive research findings, genetically engineered rat models are becoming increasingly popular. Get ahead of your competition by leveraging Applied StemCell’s expertise in CRISPR/Cas9 and TARGATT™ genome editing technologies and animal models engineering to generate the ideal rat model to meet your specifications and budget.

  • Global leader in genetically engineered rat models (Nature article, “CRISPR mouse model boom, rat model renaissance”)
  • Rat models are generated in the US in an AAALAC accredited animal facility using ISO:9001 quality standards
  • Detailed discussion to understand project requirements, optimal project design for precise genetic modification and dedicated project management for timely updates
Rat Models Categories

CRISPR Knock-In, Knock-Out,
Conditional Knock-Out Rat

CRISPR/Cas9 technology will be used to generate rat models that contain point mutation(s), small reporter/ gene insertions and conditional knockouts.

CRISPR Knock-In, Knock-Out,
Conditional Knock-Out Rat

Bacterial Artificial Chromosome (BAC) Knock-in and Conditional Knock-In Rat Models

We offer BAC design, cloning strategy, cloning and sequence validation of the BAC, pronuclear microinjection of BAC into rat embryos, and genotyping.

Bacterial Artificial Chromosome (BAC) Knock-in and Conditional Knock-In Rat Models

Technical Details

For decades, mice have been the major transgenic in vivo model for gene function studies and drug discovery. Their low maintenance cost and easy manipulation made them a preferred tool for pre-clinical research. However, there is still a significant void in animal models required for specific research purposes which the mouse models have been unable to fill. As the need for a better understanding of human genetics has grown, there has been an increased demand for improved transgenic animal models.

Rat models are making a comeback as a preferred in vivo model for researchers who seek a better representation of human genetics and physiology. The successful isolation of rat embryonic stem cells (rES) and complementary accomplishments in site-directed mutagenesis using techniques such as TARGATT™, CRISPR/Cas9, ZFNs and TALENs have made the generation of transgenic rat models possible. 

Why do we need transgenic rat models?

  • Mouse models are not always reliable in preclinical studies and have several limitations for modeling human diseases
  • The metabolism, physiology and pathology of rats are closer to human than mice
  • The larger size of rats allows for sophisticated surgeries, instrumentation and manipulations
  • Excellent behavioral models for cognition and memory, neurological and psychological assays and drug screening
  • Superior model for cardiovascular diseases (stroke and hypertension), autoimmune disorders, diabetes, breast cancer, and autoimmune disorders

 Comparison of Transgenic Technologies

Methods

Technical Advantage

TARGATT™ phiC31 integrase

  • Site specific integration (H11 or ROSA26)
  • Works for large DNA Knock-in (-22kb)

CRISPR / Cas9

  • High specificity
  • High frequency in Knockout, Conditional Knockout
  • Ease of use
  • Works for large DNA knock-in (-10kb)

New! Custom In Vivo (Animal Models) Assay Services for downstream evaluation of your animal models. We provide services for in vivo assessments as well as in vitro (end-of-study) evaluations using assays such as electrophysiology, immunohistochemistry, and more.

Have Questions?

An Applied StemCell technical expert is happy to help, contact us today!