ASC’s TARGATT™ Technology is Featured in Microinjection: Methods and Protocols (Book in the Methods in Molecular Biology Series)

1/9/19 MILPITAS, Calif.--Applied StemCell (ASC) is very excited to announce the publication of Chapter 4 in the book, Microinjection: Methods and Protocols featuring its proprietary TARGATT™ technology for site-specific gene integration. The chapter was authored by Dr. Ruby Yanru Chen-Tsai, co-founder and CSO at ASC, and co-inventor of the TARGATT™ technology.

The book itself is part of the highly successful Methods in Molecular Biology series, and explains in extensive detail about microinjection protocols, reagents, and troubleshooting tips, and the critical role it currently plays in generating animal models for biomedical research.

The TARGATT™ technology, featured in chapter 4 titled, Using TARGATT™ Technology to Generate Site-Specific Transgenic Mice, discusses the PhiC31 integrase-mediated approach to generate site-specific transgenic mice via pronuclear microinjection. The TARGATT™ integration enables integration of an intact single-copy of the transgene of interest, at a preselected genetic locus engineered with a docking-site, with high efficiency, high level expression of the transgene, and fast generation time (as fast as 3 months).

ASC has very successfully employed the TARGATT™ technology in the custom mouse model generation service platform to generate Site-Specific Knock-in Mouse Models for a variety of research applications.  Several of these mouse models have been successfully showcased in leading peer-reviewed scientific publications. For researchers experience in transgenic mouse model generation, the company also offers products including the docking-site ready TARGATT™ “attP” mice, transgenic kits and plasmids for mouse model engineering in their own animal facilities.

For further details regarding this technology and service/ product offerings, please contact ASC at


Microinjection Methods and Protocols

Edited by: Chengyu Liu and Yubin Du

National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA

ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-8830-3 ISBN 978-1-4939-8831-0 (eBook)